Continuous Separation of Breast Cancer Cells from Blood Using Multi-stage Multi-orifice Flow Fractionation (ms-moff)
نویسندگان
چکیده
This research presents an multi-stage multi-orifice flow fractionation (MS-MOFF) device designed for separating breast cancer cell from blood. The structure and dimensions of the MS-MOFF were determined by the hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating the non-selected target cells. The recovery of breast cancer cell increased from 88.8 % to over 93.3 % through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood such as circulating tumor cell solely by using hydrodynamic forces.
منابع مشابه
Development of an Ensemble Multi-stage Machine for Prediction of Breast Cancer Survivability
Prediction of cancer survivability using machine learning techniques has become a popular approach in recent years. In this regard, an important issue is that preparation of some features may need conducting difficult and costly experiments while these features have less significant impacts on the final decision and can be ignored from the feature set. Therefore, developing a machine for p...
متن کاملEffects of 12 Weeks of Aerobic Exercise (High-Intensity Interval Training or Moderate Intensity Continuous Training) with and without Blood Flow Restriction on Anthropometric Indices in Women with Cardiotoxicity after Breast Cancer Treatment
Introduction: Cardiotoxicity is an unavoidable complication after chemotherapy. The present study investigated the effect of 12 weeks of aerobic exercise (high-intensity interval training or moderate-intensity continuous training) with and without blood flow restriction on some anthropometric indices in women with breast cancer who had chemotherapy-induced cardiotoxicity. Methods: Twenty breas...
متن کاملInvestigation of NK Cell Population in Peripheral Blood and Tumor Lesions of Patients with Breast Cancer
Background: Alteration in peripheral blood lymphocytes (PBLs) is usually investigated to provide an evidence of the host immune responses to tumor antigens. The tumor infiltrating NK cells interact most closely with the tumor cells and more accurately reflect tumor host interactions. Objective: To analyze peripheral blood and tumor associated Natural Killer (NK) cells in patients with breast ca...
متن کاملCell separation by dielectrophoretic field-flow-fractionation.
Dielectrophoretic field-flow-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34+ hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped ...
متن کاملEvaluation of correlation between risk factors of breast tumors by Multi Nominal Logit Model
Breast cancer is a malignant tumor and the most common cancer among American women that starts from cells of the breast. The growth of the size of data exceeds the ability of humans to analyze the data. So we need some intelligent machines that can analyze datasets intelligently for us. We propose a Multi Nominal Logit Model to evaluate correlation between risk factors of breast tumor. Our ...
متن کامل